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Identification of Tomato Ringspot Virus in Ash in New York. C. R. Hibben, Brooklyn Botanic Garden Research Center, Ossining, NY 10562. J. A. Reese, and J. D. Castello. Brooklyn Botanic Garden Research Center, Ossining, NY 10562, and Department of Environmental and Forest Biology, State University of New York, Syracuse 13210. Plant Dis. 72:175. Accepted for publication 1 October 1987. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/PD-72-0175D.

Mature white ash (Fraxinus americana L.) on a O.1-ha woodland site in Verbank, New York, showed 10-50% branch dieback and were felled. A virus was sap-transmitted from stump sprouts from seven of25 cut ash to Vigna unguiculata (L.) Walp. Sucrose density-gradient centrifugation of partially purified virus yielded two infectious components (isometric particles about 28 nm in diameter) with estimated sedimentation coefficients of 120 and 132 S. The ash isolate was identified by serology as tomato ringspot virus (TmRSV). Young ash leaves were mechanically inoculated with purified TmRSV. Virus was detected by ELISA in eight of 88 inoculated ash after two cycles of growth and dormancy. Symptoms on three of the inoculated ash consisted of chlorotic spots, blotches, and line patterns. TmRSV was identified by ELISA in 12 of 23 ash at three sites in Onondaga County. This is the first report of TmRSV in ash and the fourth virus (I) identified in this host.

Reference: (1) J. I. Cooper and 1. B. Sweet. Forestry 49:73, 1976.