Identification of Tomato Ringspot Virus in Ash in New York. C. R. Hibben, Brooklyn Botanic Garden Research Center, Ossining, NY 10562. J. A. Reese, and J. D. Castello. Brooklyn Botanic Garden Research Center, Ossining, NY 10562, and Department of Environmental and Forest Biology, State University of New York, Syracuse 13210. Plant Dis. 72:175. Accepted for publication 1 October 1987. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/PD-72-0175D.
Mature white ash (Fraxinus americana L.) on a O.1-ha woodland site
in Verbank, New York, showed 10-50% branch dieback and were
felled. A virus was sap-transmitted from stump sprouts from seven of25
cut ash to Vigna unguiculata (L.) Walp. Sucrose density-gradient
centrifugation of partially purified virus yielded two infectious
components (isometric particles about 28 nm in diameter) with
estimated sedimentation coefficients of 120 and 132 S. The ash isolate
was identified by serology as tomato ringspot virus (TmRSV). Young
ash leaves were mechanically inoculated with purified TmRSV. Virus
was detected by ELISA in eight of 88 inoculated ash after two cycles of
growth and dormancy. Symptoms on three of the inoculated ash
consisted of chlorotic spots, blotches, and line patterns. TmRSV was
identified by ELISA in 12 of 23 ash at three sites in Onondaga County.
This is the first report of TmRSV in ash and the fourth virus (I)
identified in this host.