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Some Properties of a Strain of Cucumber Mosaic Virus Isolated from Chickpea in India. T. V. Chalam, Research Scholar, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru P.O. 502 324, Andhra Pradesh, India. M. V. Reddy, Chickpea Pathologist, International Centre for Agricultural Research in the Dry Areas, Aleppo, Syria; Y. L. Nene, Program Leader, S. P. S. Beniwal, Senior Plant Pathologist, Pulses Improvement Program, ICRISAT; and J. Subbayya, Professor of Plant Pathology, S. V. Agricultural College, Tirupati 507 502, Andhra Pradesh, India. Plant Dis. 70:128-130. Accepted for publication 12 July 1985. Copyright 1986 The American Phytopathological Society. DOI: 10.1094/PD-70-128.

A virus isolated in India from chickpea plants showing tip necrosis and mosaic was identified as a strain of cucumber mosaic virus on the basis of host range, in vitro properties, insect transmission, electron microscopy, and serology. The purified virus had a sedimentation coefficient of 104 S and a buoyant density of 1.325 g/ ml. The polyacrylamide gel electrophoresis (PAGE) of virus coat protein revealed the presence of a single polypeptide with a molecular weight of 25,000. The PAGE of virus RNA revealed the presence of four RNA species. An antiserum to the virus produced in rabbit had a titer of 1/1,024 in double-diffusion tests.