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Serological Reactivities of Endophytic Fungi from Tall Fescue and Perennial Ryegrass and of EpichloŽ typhina. Mark C. Johnson, Department of Plant Pathology, University of Kentucky, Lexington 40546. Malcolm R. Siegel, and Beth A. Schmidt, Department of Plant Pathology, University of Kentucky, Lexington 40546. Plant Dis. 69:200-202. Accepted for publication 21 August 1984. Copyright 1985 The American Phytopathological Society. DOI: 10.1094/PD-69-200.

There were few or no significant differences among absorbances elicited by mycelial preparations of six isolates of a tall fescue endophyte, an isolate of an endophyte from perennial ryegrass, and isolates of EpichloŽ typhina from bentgrass and wedgegrass when tested against an antiserum prepared to one of the tall fescue isolates (Ky-1) in a direct enzyme-linked immunosorbent assay (ELISA). Mycelial preparations of the various isolates were tested at concentrations ranging from 1 μg/ml to 1 mg/ml. All tall fescue isolates elicited absorbances that were not significantly different from each other at any given mycelial dry weight concentration. Mycelial homogenates of the endophyte from perennial ryegrass and the two isolates of E. typhina appeared to be less reactive than the isolates of tall fescue endophyte at the lower mycelial dry weight concentrations tested. Our ELISA system easily detected the perennial ryegrass endophyte in situ.