First Report of Tomato Ringspot Virus Infecting Daphne. . A. W. Chiko, Saanichton Research and Plant Quarantine Station, Sidney, B.C., Canada V8L 1H3. S. E. Godkin, Saanichton Research and Plant Quarantine Station, Sidney, B.C., Canada V8L 1H3. Plant Dis. 68:536. Accepted for publication 5 March 1984. Copyright 1984 The American Phytopathological Society. DOI: 10.1094/PD-68-536a.
A virus with spherical particles about 27 nm in diameter, isolated from mottled leaves of a Daphne mezereum L. plant growing near Victoria, B.C., was manually transmitted to 15 of 26 herbaceous species in seven of 10 families. It induced systemic apical necrosis and was seed-transmitted in Chenopodium amaranticolor Coste & Reyn. and C. quinoa/ Willd. In extract from systemically infected leaves of Petunia hybrida Vilm., the dilution end point was 10-'-10~*, the thermal inactivation point was 50-55 C, and longevity at room temperature was 6-8 days. In immuno diffusion tests, the virus reacted with antisera to tomato ringspot virus obtained from two laboratories. It did not react with antisera to four nepoviruses previously isolated from Daphne spp. or with antisera to six other nepoviruses.