Recovery of Corynebacterium sepedonicum from Sugar Beet. W. M. Bugbee, (ARS, USDA) Department of Plant Pathology, North Dakota State University, Fargo 58105. N. C. Gudmestad, G. A. Secor, and P. Nolte, Department of Plant Pathology, North Dakota State University, Fargo 58105. Plant Dis. 68:1099. Accepted for publication 4 September 1984. Copyright 1984 The American Phytopathological Society. DOI: 10.1094/PD-68-1099c.
Corynebacrerium sepedonicum (Spieck. & Kotth.) Skapt. & Burkh. was isolated from symptomless sugar beets (Beta vulqaris L.) that had been grown from seed for 6 wk in a greenhouse potting mix containing nonsterile soil. The roots were sterilized by sequential detergent wash, 1% sodium hypochlorite, and flame-off of ethanol. The sugar beet strain was morphologically and serologically indistinguishable from a potato strain and caused wilt of tomato and potato. The bacterium was reisolated from wilted tomato, reidentified as C. sepedonicum, and reinoculated by stem injection into tomato, with subsequent wilt development. The bacterium was also isolated from surface-sterilized sugar beet roots grown in pasteurized soil inoculated with homogenized potatoes with ring rot and from tomatoes transplanted into soil containing a homogenate of a field-grown sugar beet root that had been submerged in water for 3 days to initiate deterioration.