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Optimum Conditions for Studies of Maize Dwarf Mosaic Virus Strains A and B in Sorghum. Dallas L. Seifers, Research Plant Pathologist, Fort Hays Branch Experiment Station, Hays, KS 67601. Plant Dis. 68:1067-1069. Accepted for publication 15 May 1984. Copyright 1984 The American Phytopathological Society. DOI: 10.1094/PD-68-1067.

Optimum conditions for the study of maize dwarf mosaic virus strains A and B (MDMV-A and MDMV-B) in sorghum are 1) use of 1:10 and 1:2 dilutions of inoculum (MDMV-A and MDMV-B, respectively); 2) source plants inoculated 10 days before use; 3) an incubation temperature of 25 C; and 4) inoculation of the first leaf 6 days after plant emergence, of the second leaf 2 days later, and of the third leaf at least 14 days after plant emergence.