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Selective Medium for Isolating Cochliobolus sativus from Soil. Erlei Melo Reis, Centro Nacional de Pesquisa de Trigo-EMBRAPA, Caixa Postal 569, 99100 Passo Fundo, RS, Brazil. Plant Dis. 67:68-70. Accepted for publication 22 May 1982. Copyright 1983 American Phytopathological Society. DOI: 10.1094/PD-67-68.

A plate-count method using a selective medium has been devised for population studies of Cochliobolus sativus in field soils. The medium, quarter-strength potato-sucrose agar, contains antibiotics (streptomycin and neomycin) and fungicides (benomyl, captan, and dicloran). It restricts the development of undesirable fast-growing fungi, bacteria, and actinomycetes but permits growth of C. sativus. Its efficiency in determining C. sativus populations in field soils has been demonstrated using 1:100 soil dilutions in 0.1% water agar.

Keyword(s): common root rot, inoculum density.