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Effect of Storage Temperatures on Potato Virus Infectivity Levels and Serological Detection by Enzyme-Linked Immunosorbent Assay. R. P. Singh, Research Scientist, Agriculture Canada, Research Branch, Research Station, Fredericton, New Brunswick, E3B 4Z7. T. H. Somerville, Technician, Agriculture Canada, Research Branch, Research Station, Fredericton, New Brunswick, E3B 4Z7. Plant Dis. 67:1133-1136. Accepted for publication 20 April 1983. Copyright 1983 Department of Agriculture, Government of Canada. DOI: 10.1094/PD-67-1133.

Leaves infected with potato leafroll virus or potato virus A, S, X, or Y stored at 25, 4, 20, or 70 C produced variable infectivity and serological readings. Freezing at 20 C lowered the A405 values in ELISA for all potato viruses tested except PVX. Infectivity levels of PVA and PVY were also lowered at 20 C. Refrigeration of leaves at 4 C or freezing at 70 C preserved the infectivity and serological activity up to 12 days. The lower A405 values at 20 C were not due to dilution effect, were not affected by host species, and were similar for stored leaves and expressed sap. Purification of PVY from leaves stored at 4, 20, and 70 C produced the lowest yield of virus at 20 C and the highest at 70 C. Pretreatment of leaves in solutions of 5% yeast extract, 5% bactotryptone, 5% bactopeptone, 10% sucrose, or 10% glycine followed by storage at 20 C partially retained both infectivity and serological activity.