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Indexing System for Sweet Potato Feathery Mottle Virus in Sweet Potato Using Enzyme-Linked Immunosorbent Assay. P. R. Esbenshade, Graduate Student, Department of Plant Pathology, North Carolina State University, Raleigh 27650. J. W. Moyer, Associate Professor, Department of Plant Pathology, North Carolina State University, Raleigh 27650. Plant Dis. 66:911-913. Accepted for publication 21 December 1981. Copyright 1982 American Phytopathological Society. DOI: 10.1094/PD-66-911.

Enzyme-linked immunosorbent assay was adapted for detection of sweet potato feathery mottle virus in sweet potato (Ipomoea batatas). Detectable virus levels were highest in shoots 58 wk after emergence. There were highly significant differences in detectable virus levels between leaf positions sampled from shoots infected with feathery mottle virus. Leaves near the proximal portion of the shoot were the most consistent source of detectable virus. Enzyme-linked immunosorbent assay detected 100% of the plants (ie, a root and all shoots produced by that root) infected with feathery mottle virus by sampling two shoots per plant, using a composite sample of three leaves from each shoot.

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