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Selective Medium for Recovering Verticicladiella procera from Soils and Symptomatic White Pines. I. S. Swai, Former Graduate Student, Division of Plant and Soil Sciences, College of Agriculture and Forestry, West Virginia University, Morgantown 26506. D. F. Hindal, Associate Professor of Mycology, Division of Plant and Soil Sciences, College of Agriculture and Forestry, West Virginia University, Morgantown 26506. Plant Dis. 65:963-965. Accepted for publication 30 March 1981. Copyright 1981 American Phytopathological Society. DOI: 10.1094/PD-65-963.

A selective medium was developed for recovering Verticicladiella procera from infested soils and symptomatic white pines. The medium, with the pH adjusted to 4.55.0 before autoclaving, contained glucose, microelements, chlorotetracycline hydrochloride, cycloheximide, and streptomycin sulfate. The pathogen was recovered regularly when wood samples from cankers or soils from the rhizosphere of symptomatic trees were incubated on this medium for 715 days at 20 C.