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Germination and Establishment of Infection on Grape Berries by Single Airborne Conidia of Botrytis cinerea

June 2001 , Volume 85 , Number  6
Pages  668 - 677

S. Coertze and G. Holz , Department of Plant Pathology , and A. Sadie , Department of Biometry, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa

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Accepted for publication 12 March 2001.

Table grapes (cv. Dauphine) at different phenological stages were dusted in a settling tower with dry conidia of Botrytis cinerea. The berries were incubated for periods of 3 to 96 h at high relative humidity (RH; ±93% RH, moist berries), or were covered with a film of water (wet berries). Germination of the solitary conidia, appressorium formation, stilbene and suberin induction by germlings, and germling viability were examined by fluorescence microscopy after each incubation period. Isolation and freezing studies were conducted to determine surface colonization (berries left unsterile) and penetration (surface-disinfested berries). Symptoms were determined on berries incubated at a specific wetness regime, kept dry for 10 days, and then incubated for 4 days at high RH. Microscopic observations indicated that germination was delayed on immature berries, but proceeded at a high rate on mature berries. Growth was invariably restricted on moist berries. Attempted penetration was always direct. Stilbene and suberin were generally induced early and were intense on berries at the pea-size and bunch closure stages. Dieback of conidia and germlings occurred at a significantly higher rate on wet than moist berries, and was more pronounced on immature than on mature berries. The segment isolation and freezing studies showed that infections in grape berry cheeks established by this infection mode were few. Extended incubation periods did not lead to substantially higher rates of surface colonization and skin penetration. Disease symptoms did not develop during the 14-day period on the berries transferred to dry perspex chambers, irrespective of phenological stage, incubation period, or wetness regime. According to these findings, this mode of infection should not contribute to a gradual build-up of secondary inoculum in the vineyard, and to B. cinerea epiphytotics.

Additional keywords: conidial behavior, host resistance, infection mode, penetration, symptom expression

© 2001 The American Phytopathological Society