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Purification, Host Range, Serology, and Partial Sequencing of Dendrobium Mosaic Potyvirus, A New Member of the Bean Common Mosaic Virus Subgroup. J. S. Hu, Department of Plant Pathology, University of Hawaii, Honolulu 96822; S. Ferreria(2), M. Wang(3), W. B. Borth(4), G. Mink(5), and R. Jordan(6). (2)(3)(4)Department of Plant Pathology, University of Hawaii, Honolulu 96822; (5)Washington State University, Prosser 99350; (6)USDA-ARS, US National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705. Phytopathology 85:542-546. Accepted for publication 6 February 1995. Copyright 1995 The American Phytopathological Society. DOI: 10.1094/Phyto-85-542.

Dendrobium mosaic potyvirus (DeMV) was successfully transmitted from honohono orchids (Dendrobium superbum Reichb. f.) to Nicotiana benthamiana. The coat protein of the virus purified from N. benthamiana had a molecular weight of 34 103 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, and the viral RNA was approximately 9.5 kb in size in denatured agarose gel electrophoresis. With a polyclonal antiserum produced against purified DeMV particles, DeMV was found to be widespread in honohono orchids in Hawaii. Based on serological reactions in indirect enzyme-linked immunosorbent assay with monoclonal antibodies. DeMV is a potyvirus of the bean common mosaic virus (BCMV) subgroup. DeMV-specific cDNA clones were obtained and sequenced. Sequence comparison using the coat protein amino acid sequence and the 3' untranslated region sequence showed that DeMV shares 88 to 91% and 85 to 95% identity, respectively, with those sequences of BCMV subgroup members. Data from host range studies suggest that DeMV behaves similarly to other potyviruses in the BCMV subgroup such as azuki bean mosaic virus (AzMV) and blackeye cowpea mosaic virus (BICMV). Based on available information, DeMV is considered a member of the BCMV subgroup.