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Physiology and Biochemistry

Characterization of Rice Tungro Bacilliform and Rice Tungro Spherical Viruses. H. Hibino, National Agriculture Research Center, Kannondai, Tsukuba 305, Japan; K. Ishikawa(2), T. Omura(3), P. Q. Cabauatan(4), and H. Koganezawa(5). (2)(3)National Agriculture Research Center, Kannondai, Tsukuba 305, Japan; (4)(5)International Rice Research Institute, P.O. Box 933, Manila, Philippines. Phytopathology 81:1130-1132. Accepted for publication 23 April 1991. Copyright 1991 The American Phytopathological Society. DOI: 10.1094/Phyto-81-1130.

Nucleic acids and proteins of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV) were analyzed by gel electro-phoresis. RTSV contains single-stranded RNA of 3.7 106 Da and two major proteins of 24 and 23 kDa. RTBV contains circular double-stranded DNA of 8.3 kbp and a single major protein of 32 kDa. The RTBV-DNA is 2.12.3 ?m in length and has two discontinuities, one in each of the two strands, having different polarities. Endonuclease cleavage sites in the RTBV-DNA molecule are mapped.

Additional keywords: helper and dependent virus combination, leafhopper-borne viruses, rice tungro disease.