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Discrimination Among Luteoviruses and Their Strains by Monoclonal Antibodies and Identification of Common Epitopes. Cleora J. D’Arcy, Department of Plant Pathology, University of Illinois, Urbana 61801; L. Torrance(2), and R. R. Martin(3). (2)Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, United Kingdom; (3)Agriculture Canada Research Station, 6660 N.W. Marine Drive, Vancouver, British Columbia, Canada V6T 1X2. Phytopathology 79:869-873. Accepted for publication 4 April 1989. Copyright 1989 The American Phytopathological Society. DOI: 10.1094/Phyto-79-869.

Twenty-seven monoclonal antibodies to four luteoviruses were tested for their ability to detect 17 strains of seven luteoviruses in triple-antibody-sandwich enzyme-linked immunosorbent assay. Monoclonal antibodies (produced in Canada, the United Kingdom, and the United States) were to barley yellow dwarf virus (BYDV)-PAV (PAV) (3), BYDV-MAV (MAV) (3), BYDV-RPV (RPV) (5), beet western yellows virus (BWYV) (6), potato leafroll virus (PLRV) (6), and soybean dwarf virus (SDV) (4). Virus-specific monoclonal antibodies were identified for each virus to which antibodies had been produced. Many heterologous reactions, some as strong as the homologous reactions, were detected. The minimum number of epitopes identified on each virus varied from one to five. Common epitopes were identified for MAV-PAV, PAV-PLRV, PAV-PLRV-BWYV (beet mild yellowing strain), RPV-bean leafroll virus (BLRV), RPV-PLRV, RPV-BLRV-BWYV, BWYV-PLRV, BWYV-SDV, PLRV-groundnut rosette assistor virus, and PLRV-SDV. The serological relationships between PAV and PLRV and between RPV and BLRV have not been reported previously. None of the antibodies tested detected BYDV-RMV, carrot red leaf virus, or strawberry mild yellow edge virus, a possible luteovirus.