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Soilborne Wheat Mosaic Virus Antigen Binds to Cylindrical Inclusions of Potyviruses in Doubly Infected Cells of Wheat. Willem G. Langenberg, U.S. Department of Agriculture, Agricultural Research Service, and Department of Plant Pathology, University of Nebraska, Lincoln 68583; Phytopathology 79:1265-1271. Accepted for publication 22 June 1989. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1989. DOI: 10.1094/Phyto-79-1265.

Both intact virions and capsid proteins of soilborne wheat mosaic virus bound to cylindrical inclusions of the potyviruses Hordeum mosaic virus or Agropyron mosaic virus in doubly infected wheat cells but not to other cell organelles. Double labeling of ultrathin sections first with primary antiserum to one of the viruses and then with goat anti-rabbit attached to colloidal gold could precisely localize each antigen. The results confirm earlier findings that proteinaceous plates that form the cylindrical inclusions or pinwheels of potyviruses can bind heterologous as well as homologous virions or capsid proteins of rod-shaped viruses.

Additional keywords: immunogold, localization of viral proteins, viral protein attachment.