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Differentiation of Soybean Mosaic Virus Isolates by One-Dimensional Trypsin Peptide Maps Immunoblotted with Monoclonal Antibodies. J. H. Hill, Department of Plant Pathology, Iowa State University, Ames 50011; H. I. Benner(2), T. A. Permar(3), T. B. Bailey(4), R. E. Andrews, Jr.(5), D. P. Durand(6), and R. A. Van Deusen(7). (2)(3)(7)Department of Plant Pathology, Iowa State University, Ames 50011; (4)Department of Statistics, Iowa State University, Ames 50011; (5)(6)Department of Microbiology, Iowa State University, Ames 50011. Phytopathology 79:1261-1265. Accepted for publication 22 June 1989. Copyright 1989 The American Phytopathological Society. DOI: 10.1094/Phyto-79-1261.

Twelve monoclonal antibodies were used to differentiate 11 isolates of soybean mosaic virus by immunoblot analysis of one-dimensional trypsin peptide maps of the virion capsid protein. Although all isolates showed unique patterns that reflected epitopic specificity of the viral coat protein, the virus isolates formed three distinct groups. These results suggest potential for using this approach to study antigenic drift and plant virus epidemiology.

Additional keywords: potyviruses, serology.