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Molecular Plant Pathology

Double-Stranded RNA Molecules from Michigan Hypovirulent Isolates of Endothia parasitica Vary in Size and Sequence Homology. C. P. Paul, Graduate research assistant, Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312; D. W. Fulbright, Associate professor, Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312. Phytopathology 78:751-755. Accepted for publication 11 December 1987. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-751.

The sizes and homology relationships among double-stranded RNA (dsRNA) molecules from American and European hypovirulent isolates of Endothia parasitica were compared. The number, size, and relative intensity of dsRNA bands in gels varied among isolates. The sizes of dsRNA segments from each isolate ranged from a molecular weight of 0.56 106 to 6.2 106. No single size of dsRNA molecule was common to all isolates. The dsRNA from isolate GH2 hybridized to dsRNA from all other Michigan isolates tested, with the exception of dsRNA from isolate RC1, RC1 dsRNA did not hybridize to dsRNA from any other Michigan isolates tested. Neither GH2 nor RC1 dsRNA shared homology with dsRNA from other states or from Europe.

Additional keywords: chestnut blight, Cryphonectria parasitica.