Previous View
APSnet Home
Phytopathology Home


Molecular Plant Pathology

Isolation, Molecular Cloning, and Detection of Strawberry Vein Banding Virus DNA. D. C. Stenger, Graduate research assistant, Department of Plant Pathology, University of California, Berkeley 94720; R. H. Mullin, and T. J. Morris. Staff research associate, and Professor, respectively, Department of Plant Pathology, University of California, Berkeley 94720. Phytopathology 78:154-159. Accepted for publication 24 July 1987. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-154.

DNA with caulimovirus properties was isolated from strawberry vein banding virus (SVBV). Native viral DNA of 7.8 kbp was circular and double-stranded. Each DNA strand contained one discontinuity positioned at either 0.0 or 0.5 map units on the circular molecule. EcoRI-digested SVBV DNA was cloned into the Escherichia coli plasmid pUC8. A recombinant plasmid (pSVBV-E3) containing a 7.8-kbp EcoRI insert hybridized to SVBV DNA but not to cauliflower mosaic virus DNA and had a restriction map identical to that of SVBV DNA. Dot hybridization tests using pSVBV-E3 as a probe indicated SVBV DNA titer varied greatly between leaflets sampled from the same plant.