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Physiology and Biochemistry

Characterization of the Peanut Mottle Virus Genome by in Vitro Translation. Z. Xiong, Department of Plant Pathology, University of Florida, Gainesville 32611; E. Hiebert, and D. E. Purcifull. Department of Plant Pathology, University of Florida, Gainesville 32611. Phytopathology 78:1128-1134. Accepted for publication 5 April 1988. Copyright 1988 The American Phytopathological Society. DOI: 10.1094/Phyto-78-1128.

Peanut mottle virus (PMoV) RNA was translated in the rabbit reticulocyte lysate (RRL) and wheat germ systems. The translation products were analyzed by immunoprecipitation with antisera to several potyviral proteins. Comparisons were made between the RRL products translated in the absence and presence of dithiothreitol (DTT) and between the translation products formed in the absence of DTT subjected to subsequent incubation in the absence and presence of DTT. Proteolytic processing of the in vitro translation products was apparent. In the absence of DTT, PMoV RNA translation in RRL produced Mr 84,000 (84K) and 210250K polyproteins, which were subsequently cleaved into smaller proteins during incubation with DTT. Based on the analysis of the in vitro translation products by antisera to potyviral proteins and by the precursor-product relationships observed during manipulation of the proteolytic processing, a PMoV genomic map was proposed from 5 to 3 termini as follows: 34K unknown protein, 50K helper component protein, 42K unknown protein, 68K cylindrical inclusion protein, 50K nuclear inclusion protein, 53K nuclear inclusion protein, and 34K coat protein.