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Production of Monoclonal Antibodies to Peanut Mottle Virus and Their Use in Enzyme-Linked Immunosorbent Assay and Dot-Immunobinding Assay. J. L. Sherwood, Assistant professor, Department of Plant Pathology, Oklahoma State University, Stillwater 74078; M. R. Sanborn(2), and G. C. Keyser(3). (2)Associate professor, Department of Botany and Microbiology, Oklahoma State University, Stillwater 74078; (3)Administrative and professional, Department of Plant Pathology, Oklahoma State University, Stillwater 74078. Phytopathology 77:1158-1161. Accepted for publication 19 February 1987. Copyright 1987 The American Phytopathological Society. DOI: 10.1094/Phyto-77-1158.

Stable hybridoma cell lines secreting monoclonal antibodies (MAB) to peanut mottle virus (PMV) were produced by fusing spleen cells of immunized mice and mouse myeloma cell line P3X63Ag8.653. Hybridoma clones produced antibodies of the IgG1 subclass. The MAB reacted to nine isolates of PMV but did not react to eight other viruses tested. The immunoreactivity of the MAB was compared with polyclonal rabbit serum for detection of PMV in foliar tissue by enzyme-linked immunosorbent assay (ELISA) and a dot-immunobinding assay. An ELISA using both polyclonal antibodies and MAB was superior to assays using either source of antibody alone. However, a dot-immunobinding assay using only MAB was satisfactory for detection of PMV.

Additional keywords: polyclonal antiserum, potyvirus.