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Differences Among Monoclonal Antibodies to Barley Yellow Dwarf Viruses. H. T. Hsu, American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD 20852; J. Aebig(2), and W. F. Rochow(3). (2)American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD 20852; (3)ARS, U.S. Department of Agriculture, Plant Pathology Department, Cornell University, Ithaca, NY 14853. Phytopathology 74:600-605. Accepted for publication 30 November 1983. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1984. DOI: 10.1094/Phyto-74-600.

Monoclonal antibodies of hybridoma clones derived from mice injected with either the RPV or MAV isolate of barley yellow dwarf virus (BYDV) were evaluated in a variety of tests with five previously characterized BYDV luteoviruses. Five cell lines were from mice injected with RPV, and eight lines were from MAV-injected mice. Three of the 13 cloned hybridomas ceased producing antibodies; three others produced antibodies that reacted with healthy oat antigen. Of the seven virus-specific antibodies, which represented four antibody isotypes, three reacted only with RPV and one only with MAV. Two additional MAV-derived antibodies reacted with both MAV and PAV. One reacted with MAV and SGV. Specificity of the reactions was consistent in two kinds of enzyme-linked immunosorbent assays and in preliminary neutralization studies carried out in test tubes or within aphid vectors. The potential value of these monoclonal antibodies for use in luteovirus research is discussed.

Additional keywords: cell hybridization, murine monoclonal antibodies, neutralization, somatic techniques.