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Physiology and Biochemistry

Further Characterization of Panicum Mosaic Virus and Its Associated Satellite Virus. F. G. Buzen, Jr., Former research assistant, Department of Plant Pathology, Kansas State University, Manhattan 66506 (now biologist, Olin Corporation, P.O. Box 991, Little Rock, AR 72203); C. L. Niblett(2), G. R. Hooper(3), J. Hubbard(4), and M. A. Newman(5). (2)Former associate professor, Department of Plant Pathology, Kansas State University, Manhattan 66506 (now professor, Plant Pathology Department, University of Florida, Gainesville 32611); (3)Former professor of Entomology, Michigan State University, East Lansing 48824 (now professor, Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg 24061); (4)Chemist, U.S. Grain Marketing Research Laboratory, 1515 College Ave., Manhattan, KS 66502; (5)Extension plant pathologist, University of Tennessee, 605 Airways Blvd., Jackson 38301. Phytopathology 74:313-318. Accepted for publication 20 May 1983. Copyright 1984 The American Phytopathological Society. DOI: 10.1094/Phyto-74-313.

Panicum mosaic virus (PMV) replicates in the absence of satellite panicum mosaic virus (SPMV), whereas SPMV requires PMV for its replication. PMV and SPMV are unrelated serologically. Presently, two serotypes of SPMV and six of PMV have been differentiated on the basis of their reactions with homologous and heterologous antisera. A serological relationship exists between PMV and members of the phleum mottle virus (PhMV) group that were tested. The PMV serotypes were classified into two groups based on their relative electrophoretic mobility. SPMV was found associated with all PMV serotypes tested. It was not associated with MSV. Both PMV-type and MSV were capable of directing the replication of SPMV. PMV differs from SPMV in particle size, RNA content and base composition, capsid protein molecular weight, and amino acid composition. The in vitro translation products for PMV and SPMV were compared. PMV RNA directed the synthesis of several ¹⁴C-labeled proteins, including one that coelectrophoresed with authentic PMV coat protein. SPMV RNA directed the synthesis of a single ¹⁴C-labeled protein which coelectrophoresed with its authentic coat protein. Although the relationship between PMV and SPMV mimics the relationship between tobacco necrosis virus and its satellite virus, no serological relationships were found among these four viruses.