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Physiology and Biochemistry

Comparison of Anastomosis Groups of Rhizoctonia solani by Polyacrylamide Gel Electrophoresis of Soluble Proteins. Monica Reynolds, Research assistant, Department of Plant Pathology, University of California, Berkeley 94720, Current address of senior author: Abbott Laboratories, North Chicago, IL; A. R. Weinhold(2), and T. J. Morris(3). (2)(3)Professor, and associate professor, respectively, Department of Plant Pathology, University of California, Berkeley 94720. Phytopathology 73:903-906. Accepted for publication 7 January 1983. Copyright 1983 The American Phytopathological Society. DOI: 10.1094/Phyto-73-903.

Soluble proteins were extracted from isolates of the five anastomosis groups (AG) of Rhizoctonia solani and compared by electrophoresis in polyacrylamide gels. The protein profiles for the five AG were distinctive and could be distinguished from one another. Particular emphasis was placed on determining the variability of protein patterns of isolates belonging to AG3 and AG4 from different hosts and geographic locations. The protein patterns from isolates belonging to AG3 were uniformly distinct, regardless of isolate source. Protein patterns of isolates belonging to AG4 varied in minor bands, but the general pattern was recognizable as AG4. It is suggested that polyacrylamide gel electrophoresis of soluble proteins can be used to help distinguish anastomosis groups of R. solani.

Additional keywords: Gossypium hirsutum, Solanum tuberosum, Thanatephorus cucumeris.