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Etiology

Identification of Double-Stranded RNAs Associated with Barley Yellow Dwarf Virus Infection of Oats. F. E. Gildow, Assistant professor, Department of Plant Pathology, University of California, Berkeley 94720; M. E. Ballinger(2), and W. F. Rochow(3). (2)Staff research associate, Department of Plant Pathology, University of California, Berkeley 94720; (3)Research plant pathologist, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, and professor of Plant Pathology, Cornell University, Ithaca, NY 14853. Phytopathology 73:1570-1572. Accepted for publication 6 June 1983. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1983. DOI: 10.1094/Phyto-73-1570.

Double-stranded RNAs were identified in preparations from Coast Black oats infected with each of five characterized isolates of barley yellow dwarf virus (BYDV). Differences both in the number and electrophoretic mobility of isolate-associated double-stranded RNAs (dsRNAs) support the division of BYDV isolates into two distinct groups consisting of group 1 isolates, MAV, PAV, and SGV, and group 2 isolates, RPV and RMV. Five dsRNAs with molecular weights of approximately 3.6, 2.0, 1.2, 0.55, and 0.50 x 10⁶ daltons were observed from oats infected with group 1 isolates. Only four dsRNAs with molecular weights of 3.8, 1.6, 1.2, and 0.55 x 10⁶ were detected in oats infected with group 2 isolates.