VIEW ARTICLE

Etiology

Some Properties of an Isolate of the Soybean Stunt Strain of Cucumber Mosaic Virus. K. Hanada, Plant pathologists, Institute for Plant Virus Research, Tsukuba Science City, Ibaraki, 305, Japan; H. Tochihara, Plant pathologists, Institute for Plant Virus Research, Tsukuba Science City, Ibaraki, 305, Japan. Phytopathology 72:761-764. Accepted for publication 29 September 1981. Copyright 1982 The American Phytopathological Society. DOI: 10.1094/Phyto-72-761.

An isolate of soybean stunt virus (SSV-A) obtained from mottled soybean seeds was characterized and compared with isolates of cucumber mosaic virus (CMV). SSV-A had a similar host range to that of several CMV strains, although some differences were detected. In agar gel diffusion tests, spur formation was observed between purified SSV-A and two serologically different CMVs. Polyacrylamide gel electrophoresis (PAGE) showed that SSV-A contained five major RNA species (RNAs) that had almost the same molecular weights of CMV-RNAs. A mixture of the three largest RNAs of SSV-A had the highest infectivity. SSV-A had one kind of protein with a molecular weight of ~24,000, which comigrated with CMV-protein in PAGE. Pseudorecombinants were constructed between SSV-A and CMV by exchange of RNA 3. It was concluded that SSV-A is a soybean strain of CMV with a unique serotype.