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Efficacy of Colloidal Gold-Labeled Antibody as Measured in a Barley Stripe Mosaic Virus-Lectin-Antilectin System. L. Giunchedi, Istituto di Patologia Vegetale, University of Bologna, 40126 Bologna, Italy; W. G. Langenberg, Agricultural Research Service, U.S. Department of Agriculture, and Department of Plant Pathology, University of Nebraska, Lincoln 68583. Phytopathology 72:645-647. Accepted for publication 1 September 1981. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1981.. DOI: 10.1094/Phyto-72-645.

By using colloidal gold-labeled rabbit antibody to a barley stripe mosaic virus-precipitating barley lectin isolated from barley seed, the lectin was shown to be distributed along the entire surface of the virus particle and not at the ends only. Upon standing, the specific activity of rabbit antilectin antibody labeled with colloidal gold was gradually lost by flocculation; it should be used within 1 wk after being labeled. The function of the carbohydrate portion of barley stripe mosaic virus protein remains to be elucidated.