VIEW ARTICLE

Techniques

Isolation of Specific Antibody to Plant Viruses by Acid Sucrose Density Gradient Centrifugation. M. R. McLaughlin, Former graduate research assistant, Department of Plant Pathology, University of Illinois, Urbana 61801; G. R. Bryant(2), J. H. Hill(3), H. I. Benner(4), and D. P. Durand(5). (2)(5)Graduate research assistant and professor, Department of Bacteriology, Iowa State University, Ames 50011; (3)(4)Associate professor and Laboratory Technician III, Department of Plant Pathology, Seed and Weed Sciences, Iowa State University, Ames. Phytopathology 70:831-834. Accepted for publication 4 March 1980. Copyright 1980 The American Phytopathological Society. DOI: 10.1094/Phyto-70-831.

Antibody preparations specific to southern bean mosaic (cowpea strain) and soybean mosaic viruses were isolated by reaction of virus antigen with immune serum followed by acid dissociation of immune complexes. Dissociated entities were separated by using acid sucrose density gradient centrifugation to prepare immunologically reactive specific antibody. Antibody to soybean mosaic virus was electrophoretically pure and remained reactive after lyophilization.