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Disease Detection and Losses

Detection of Barley Yellow Dwarf Virus by Enzyme-Linked Immunosorbent Assay. R. M. Lister, Professor, Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907; W. F. Rochow, research plant pathologist, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, and also professor of Plant Pathology, Cornell University, Ithaca, NY 14853. Phytopathology 69:649-654. Accepted for publication 4 January 1979. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1979. DOI: 10.1094/Phyto-69-649.

Parallel tests with aphid-transfer techniques and enzyme-linked immunosorbent assay (ELISA) indicated that ELISA was sensitive enough to detect barley yellow dwarf virus in oat leaf extracts. The reaction was specific enough to distinguish easily RPV and MAV, two distinctive vector-specific isolates of the virus. A third type of isolate, PAV, sometimes reacted, usually weakly, in heterologous tests with MAV antiserum. ELISA appears to have potential for simplifying survey and assay work with this phloem-restricted luteovirus.