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Physiology and Biochemistry

Production of Synnemata on Defined Agar Media and Its Relation to Pathogenicity of Ceratocystis ulmi. D. F. Hindal, Assistant Professor of Mycology, Division of Plant Sciences, West Virginia University, Morgantown, WV 26506; W. L. MacDonald, Associate professor, of Plant Pathology, Division of Plant Sciences, West Virginia University, Morgantown, WV 26506. Phytopathology 68:571-575. Accepted for publication 19 October 1977. Copyright 1978 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-68-571.

Defined agar media were developed which were suitable for synnema production by some isolates of Ceratocystis ulmi. Several media supported synnema production, but the best medium contained (per liter): 2.0 g glucose, 0.08 g L-asparagine, macro- and microelements, pyridoxine, and 0.50 g linoleic acid. Production of synnemata on this medium varied among isolates of C. ulmi with known pathogenicities, but more aggressive isolates generally produced more synnemata than less aggressive ones.

Additional keywords: stearic acid, oleic acid, C:N ratio.