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An Immunofluorescence Test for Maize Chlorotic Dwarf Virus. Roy E. Gingery, Research Chemist, Science and Education Administration (SEA), U.S. Department of Agriculture, Department of Plant Pathology, Ohio Agricultural Research and Development Center, Wooster, OH 44691; Phytopathology 68:1526-1529. Accepted for publication 24 April 1978. This article is in the public domain and not copyrightable. It may be freely reprinted with customary crediting of the source. The American Phytopathological Society, 1978.. DOI: 10.1094/Phyto-68-1526.

Maize chlorotic dwarf was diagnosed by indirect labeling with fluorescent antibody. Maize chlorotic dwarf virus (MCDV) was precipitated from clarified extracts with MCDV antiserum and the precipitate was labeled with fluorescein-conjugated rabbit gamma globulin antiserum. Immunofluorescence results were in good agreement with other methods for MCDV diagnosis.

Additional keywords: fluorescence microscopy, disease diagnosis.