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Location of Curly Top Virus Antigen in Bean, Sugarbeet, Tobacco, and Tomato by Fluorescent Antibody Staining. D. L. Mumford, Plant Pathologist, Agricultural Research Service, U. S. Department of Agriculture, Crops Research Laboratory, Utah State University, Logan, UT 84322; W. R. Thornley, Plant Pathologist, Immunologist, Aerostatics Instrumentation and Research, Inc., Logan, UT 84321. Phytopathology 67:1313-1316. Accepted for publication 11 April 1977. Copyright 1977 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-67-1313.

The fluorescent antibody staining method was used to locate curly top virus antigen in bean, sugarbeet, tobacco, and tomato. Antigen was found only in phloem tissue. The occurrence of specific staining corresponded to the appearance of foliar symptoms. Susceptible tomato plants had more than eight times as many specifically stained sites as did resistant plants. No localized staining was observed in viruliferous leafhoppers although a generalized fluorescence was detected.

Additional keywords: serology, fluorescein, fluorescence microscopy, conjugates, resistance.