VIEW ARTICLE

Techniques

Application of Interference Microscopy to the Study of Fungal Penetration of Epidermal Cells. Shigeyuki Mayama, Department of Botany, Southern Illinois University, Carbondale, IL 62901, Senior author present address: Laboratory of Plant Pathology, Kyoto University, Kyoto, Japan; A. J. Pappelis, Department of Botany, Southern Illinois University, Carbondale, IL 62901. Phytopathology 67:1300-1302. Accepted for publication 23 March 1977. Copyright © 1977 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-67-1300.

Penetration of outer epidermal cells of susceptible white onion leaf bases by Botrytis allii was accompanied by halo formation. Halos were visible using interference microscopy due to dry mass loss in the cell wall around the penetration site. Halos also were visible using cell wall stains. Reaction mass (dry mass deposition) was observed below penetration sites in resistant cells of red onions.