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Physiology and Biochemistry

Germination Self-Inhibitor from Cronartium comandrae Aeciospores. Deborah A. Eppstein, Former Research Assistant, Department of Plant Pathology, University of Arkansas, Fayetteville 72701; F. H. Tainter, Associate Professor, Department of Plant Pathology, University of Arkansas, Fayetteville 72701. Phytopathology 66:1395-1397. Accepted for publication 24 May 1976. Copyright © 1976 The American Phytopathological Society, 3340 Pilot Knob Road, St. Paul, MN 55121. All rights reserved.. DOI: 10.1094/Phyto-66-1395.

An unidentified compound associated with the self-inhibitory action of germinating aeciospores of Cronartium comandrae was extracted with 0.02 M ammonium bicarbonate. The inhibitor was partially purified by partition into chloroform and further purified by thin-layer chromatography. When added to suspensions of inhibitor-free germinating aeciospores, the purified inhibitor prevented germ tube emergence, but had no apparent effect when added immediately after visible germ tube emergence. Some of its physical and chemical properties are presented.