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A Severe Strain of Tobacco Mosaic Virus from Cactus. Lallan Giri, Department of Botany, University of Montana, Missoula 59801; M. Chessin, Department of Botany, University of Montana, Missoula 59801. Phytopathology 65:824-825. DOI: 10.1094/Phyto-65-824.

A previously unreported strain of tobacco mosaic virus was found in the Beavertail cactus, Opuntia basilaris, growing in the wild. Host reactions were most like those induced by the yellow-mottling distorting-type and J14D1 strains in Nicotiana sylvestris and Turkish tobacco, respectively. In crude sap, the thermal inactivation temperature was 85-90 C, and the dilution end point 108 109. Long needles were observed in polyethylene glycol precipitates of infected sap. Electron microscopy of purified virus revealed rigid rods typical of TMV with an NML of 302±5 nm. A sedimentation coefficient of 183S was determined. The virus was serologically related to common TMV.

Additional keywords: needles, premature collapse.