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Effect of Low Concentrations of Xanthomonas vesicatoria Infiltrated into Pepper Leaves. A. A. Cook, Plant Pathology Department, University of Florida, Gainesville 32611; Phytopathology 65:487-489. Accepted for publication 26 November 1974. DOI: 10.1094/Phyto-65-487.

Infiltration of Capsicum annuum leaves with pathotypes of Xanthomonas vesicatoria below the concentration required to induce visible hypersensitive response predisposed such leaves to increased electrolyte loss following reinfiltration with the same or greater bacterial concentration. The increase in electrolyte loss, over the control, was inversely related to concentration of bacteria in the second inoculation. Enhanced electrolyte loss was neither dependent on reinfiltration of inoculated leaves with the same bacterial pathotype nor consistently affected by temperature or light during incubation. The influence of reinoculation was effective between 6 and 42 hours after the first inoculation. Reinfiltration with water alone was nearly as effective in accentuating electrolyte loss as inocula containing live bacteria, but the effect of reinfiltration was reduced by calcium nitrate.