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Staining Reactions of the Tissue Bordering Lesions Induced by Wounding, Tobacco Mosaic Virus, and Tobacco Necrosis Virus in Bean. G. Faulkner, Graduate Research Assistant, Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada, Present address of senior author: Electron Microscopy Unit, Sir Charles Tupper Medical Bldg., Dalhousie University; W. C. Kimmins, Professor, Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada. Phytopathology 65:1396-1400. Accepted for publication 25 June 1975. DOI: 10.1094/Phyto-65-1396.

Necrotic lesions were produced on the primary leaves of Phaseolus vulgaris 'Pinto' and 'Prince' by inoculation with tobacco mosaic and tobacco necrosis viruses, and through chemically and mechanically induced wounding. During lesion development histochemical tests were made to determine whether changes in the cell wall composition occurred in the associated tissue. Positive reactions were noted for lignin, suberin, and callose in cells at the border of mechanical lesions and at later periods for virus lesions. While changes in cell wall composition were recorded in tissue adjacent to localized virus infections, the staining reactions were difficult to interpret and to relate to any resistance mechanism.

Additional keywords: localization, cell wall.