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Purification of Curly Top Virus. David L. Mumford, Plant Pathologist, USDA Agricultural Research Service, Crops Research Laboratory, Logan, Utah 84322; Phytopathology 64:136-139. Accepted for publication 9 July 1973. DOI: 10.1094/Phyto-64-136.

Curly top virus was purified from ‘Turkish’ tobacco. Extracts were clarified with chloroform and butanol. The virus was concentrated, either by precipitation with polyethylene glycol and NaCl, or by ultrafiltration. Clarified virus was partially purified on sucrose-density gradients, then further purified by gel chromatography, using agarose. Purified preparations had max ultraviolet-light absorption at 260 nm and min absorption at 240 nm and contained isometric particles ca. 20 nm in diameter. The virus was followed through the purification procedure by a plant-infectivity bioassay in which the insect vector fed on the infectious preparations.

Additional keywords: density-gradient centrifugation, gel chromatography, electron microscopy, sugarbeet.