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Purification of Mycoplasma-Like Organisms from China Aster Plants Affected with Clover Phyllody. R. C. Sinha, Chemistry and Biology Research Institute, Canada Agriculture, Ottawa, Ontario, Canada; Phytopathology 64:1156-1158. DOI: 10.1094/Phyto-64-1156.

Mycoplasma-like organisms (MLO) were purified from China aster (Callistephus chinensis Nees) plants infected with clover phyllody agent (CPA). Sections of pellets from purified preparations showed MLO that were very similar to those observed earlier in sections of infected plants. When healthy vector leafhoppers Macrosteles fascifrons Stal were injected with the purified preparations, and the injected insects were tested in groups of 10 per plant for their ability to transmit CPA on aster seedlings, 5 out of 50 inoculated plants became infected.