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Partial Purification of the Pea Seed-Borne Mosaic Virus. W. R. Stevenson, Research Assistant, Department of Plant Pathology, University of Wisconsin, Madison 53706; D. J. Hagedorn, Professor, Department of Plant Pathology, University of Wisconsin, Madison 53706. Phytopathology 63:1346-1352. Accepted for publication 17 April 1973. DOI: 10.1094/Phyto-63-1346.

The pea seed-borne mosaic virus (PSbMV) was partially purified using a system of high molarity buffer, chloroform, and low-speed centrifugation for initial clarification of infected pea extracts prior to polyethylene glycol (PEG-MW 6,000) precipitation and two cycles of differential ultracentrifugation for the concentration of particles. Final extracts were infectious to pea when diluted to an OD260 of 1 × 105. Electron micrographs of shadowed preparations showed high concentrations of virus particles in the partially purified product. Although repeated attempts to eliminate additional host contaminants were unsuccessful, the final preparations appeared to be suitable for use in antiserum preparation.

Additional keywords: clarification, virus concentration, aggregation.