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Comparative Gel-Electrophoresis Studies of Ribosomal Proteins of Erwinia Species and Members of the Enterobacteriaceae. C. I. Kado, Department of Plant Pathology, University of California, Davis 95616; N. W. Schaad(2), and M. G. Heskett(3). (2)(3)Department of Plant Pathology, University of California, Davis 95616, (2)Present address: Department of Plant Pathology, University of Georgia, Experiment 30212. Phytopathology 62:1077-1082. Accepted for publication 10 April 1972. DOI: 10.1094/Phyto-62-1077.

Proteins of 70 Svedbergs (S) ribosomes from Erwinia amylovora, E. carotovora, E. herbicola, Escherichia coli, and Salmonella typhimurium were analyzed by electrophoresis on acrylamide-urea gels, pH 4.3. The erwinias possess typical 70-S ribosomes which dissociate into 50-S and 30-S subunits at low magnesium concentration. The Erwinia ribosomal protein patterns are strikingly similar to those of E. coli and S. typhimurium. A characteristic feature of gel electropherograms of all these species is that the proteins cluster into two major electrophoretic groups of six to nine bands each. The gel electropherograms differ from that of Agrobacterium tumefaciens. The implications of the results are discussed.

Additional keywords: ribonuclease-1 deficient mutant of Erwinia rubrifaciens; linear transport.