Previous View
APSnet Home
Phytopathology Home


A Selective Medium for Pectolytic Fluorescent Pseudomonads. D. C. Sands, Department of Plant Pathology and Botany, The Connecticut Agricultural Experiment Station, New Haven 06504; L. Hankin(2), and M. Zucker(3). (2)(3)Departments of Plant Pathology and Botany, and of Biochemistry, respectively. The Connecticut Agricultural Experiment Station, New Haven 06504, (3)Present address: Department of Agricultural Chemistry, Washington State University, Pullman 99163. Phytopathology 62:998-1000. Accepted for publication 8 March 1972. DOI: 10.1094/Phyto-62-998.

A solid agar medium selective for fluorescent pseudomonads also shows whether the organism is pectolytic. The medium, buffered at pH 7.0, contains pectin, proteose-peptone, mineral salts, and the antibiotics novobiocin, penicillin, and cycloheximide. After growth of the organisms and determination of fluorescence, the plates are flooded with hexadecyltrimethylammonium bromide to detect zones of pectolysis. Pectolytic, fluorescent pseudomonads can be plated directly from nature due to the selectivity of the medium.

Additional keywords: Pseudomonas fluorescens, P. marginalis, pectolytic enzymes, selective medium.