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Culture of a Mycoplasmalike Organism Associated with Stubborn Disease of Citrus. Abd El -Shafy A. Fudl-Allah, Postgraduate Research Plant Pathologist, Department of Plant Pathology, University of California, Riverside 92502; E. C. Calavan(2), and E. C. K. Igwegbe(3). (2)(3)Professor, and Postgraduate Research Plant Pathologist, respectively, Department of Plant Pathology, University of California, Riverside 92502. Phytopathology 62:729-731. Accepted for publication 4 February 1972. DOI: 10.1094/Phyto-62-729.

A pleomorphic organism was cultured from young leafy shoots and seeds of stubborn-diseased citrus in cell-free media. Colonies of the “fried-egg” type were obtained on agar media, and electron micrographs of pelleted material from liquid cultures showed slender filaments and round, ovoid, or irregular mycoplasmalike bodies similar to those present in sieve tubes of infected citrus. Filaments and bodies had unit membranes about 10 nm thick.