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Development of a Selective Medium for Pseudomonas solanacearum. Asuncion D. Karganilla, Department of Plant Pathology, University of The Philippines, College, Laguna, The Philippines, and University of Hawaii, Honolulu 96822; I. W. Buddenhagen, Department of Plant Pathology, University of The Philippines, College, Laguna, The Philippines, and University of Hawaii, Honolulu 96822. Phytopathology 62:1373-1376. Accepted for publication 7 June 1972. DOI: 10.1094/Phyto-62-1373.

A selective medium consisting of a simplified basal medium plus antimicrobial compounds was developed for isolation of Pseudomonas solanacearum from the soil. Colony counts of P. solanacearum on this medium were reduced by 22-33%, as compared to a 65-99% reduction of other soil bacteria. Variation in efficiency occurred with different soil types and with the same soil held at different soil moisture tensions.