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Local Lesion Formation in Tobacco Tissue Culture. R. N. Beachy, Graduate Assistant, Department of Botany and Plant Pathology, Michigan State University, East Lansing 48823; H. H. Murakishi, Professor, Department of Botany and Plant Pathology, Michigan State University, East Lansing 48823. Phytopathology 61:877-878. DOI: 10.1094/Phyto-61-877.

Callus cultures derived from Nicotiana glutinosa and N. tabacum, ‘NN Samsun’, ‘NN Burley’, and ‘Xanthi-nc’ responded with reddish-brown local lesions when infected with tobacco mosaic virus (TMV). Cultured cells of NN Burley inoculated with 0.083 to 83 µg TMV/ml gave a virus dilution curve similar to that obtained with intact leaves of Xanthi-nc tobacco plants. Lesion formation on tissue cultures occurred only when cultures were grown on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, and coconut water.