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Immunodiffusion Assay for Potato Virus M Infection. J. F. Shepard, Department of Botany and Microbiology and Department of Veterinary Science, Montana State University, Bozeman 59715; J. W. Jutila(2), J. E. Catlin(3), F. S. Newman(4), and W. H. Hawkins(5). (2)(3)(4)(5)Department of Botany and Microbiology and Department of Veterinary Science, Montana State University, Bozeman 59715. Phytopathology 61:873-874. DOI: 10.1094/Phyto-61-873.

Ouchterlony double-immunodiffusion and single radial-diffusion procedures were successfully applied to the serodiagnosis of potato virus M (PVM). The addition of pyrrolidine to a final concentration of 2.5% to infective potato leaf extracts provided for the production of diffusible test antigen. The procedure was dependent upon the use of antibody specific for PVM pyrrolidine-degraded protein. Antiserum production was more successful in a goat than in rabbits. Goat antiserum exhibited much higher homologous antibody levels and was obtained in greater amounts.

Additional keywords: serology, goat immunization.