VIEW ARTICLE

Distribution of Tobacco Mosaic Virus in Etiolated Tobacco Leaf Cells Infected with two Viruses. Y. Honda, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan; C. Matsui, Plant Pathology Laboratory, Faculty of Agriculture, Nagoya University, Nagoya, Japan. Phytopathology 61:759-762. Accepted for publication 19 January 1971. DOI: 10.1094/Phyto-61-759.

Etiolated leaf cells of Nicotiana tabacum were studied by electron microscopy after inoculation with cucumber mosaic virus and tobacco mosaic virus (TMV). In addition to TMV particles within the cytoplasm, large and small amounts of rod-shaped particles were observed within the matrix of the nuclei and the proplastids, respectively. The rod-shaped particles within the nuclei were oriented at random, whereas the particles within the proplastids were arranged parallel to each other. Since their diameters, as well as similar particles in the cytoplasm, were about 8-9 nm, they were believed to be TMV particles. They were probably formed within the nucleus and proplastid, respectively. The rod-shaped particles could not be detected within the nuclei and plastids of TMV-infected leaf cells grown in daylight.