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Tobacco Streak Virus Isolated from Strawberry Infected with Necrotic Shock. R. Stace -Smith, Plant Pathologist, Canada Agriculture Research Station, Vancouver 8, British Columbia; N. W. Frazier, Entomologist, University of California, Berkeley 94720. Phytopathology 61:757-758. Accepted for publication 4 September 1970. DOI: 10.1094/Phyto-61-757.

Ground leaf tissue of Fragaria vesca, infected with necrotic shock by grafting, produced local lesions when it was applied to leaves of Gomphrena globosa. Purification techniques resulted in preparations having two components with sedimentation coefficients of approximately 80 S and 89 S. The 80 S component has a minimum absorption at 245 nanometers (nm) and maximum at 260 nm. The 260:280 ratios were from 1.39 to 1.43, and the particles were 30 ± 1 nm in diameter. Antiserum from rabbits had a specific titer of 1:32 against the virus. It reacted strongly with the red node strain of tobacco streak virus, indicating that the two viruses were serologically closely related if not identical. The virus was not transmitted from herbaceous hosts back to strawberry.