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Isolation of Tobacco Ringspot Virus from Rose. G. L. McDaniel, Graduate Research Assistant, Department of Horticulture, Iowa State University, Ames 50010; G. J. Buck(2), and R. E. Ford(3). (2)(3)Associate Professor, Department of Horticulture, and Professor, Department of Botany and Plant Pathology, respectively, Iowa State University, Ames 50010. Phytopathology 61:45-49. Accepted for publication 5 August 1970. DOI: 10.1094/Phyto-61-45.

Tobacco ringspot virus (TRSV) was isolated from petals of roses with line-pattern mosaic leaf symptoms. TRSV was purified from tobacco tissue by the chloroform-butanol differential centrifugation method. Host range, physical properties, and serological properties confirmed the identity of the virus, which has previously been called rose line-pattern mosaic virus and is listed in the rose mosaic virus (RMV) group according to symptoms. The virus was not serologically related to RMV, cherry necrotic ringspot virus, or tomato ringspot virus.