VIEW ARTICLE

Purification and Serology of Maize Dwarf Mosaic and Sugarcane Mosaic Viruses. T. E. Snazelle, Plant Pathologist, ARS, USDA, Lafayette, Indiana 47907, Present address of senior author: Life Science Department, Rock Valley College, Rockford, Illinois 61101; J. B. Bancroft(2), and A. J. Ullstrup(3). (2)(3)Professors of Plant Pathology, Purdue University, respectively, Lafayette, Indiana 47907. Phytopathology 61:1059-1063. Accepted for publication 2 April 1971. DOI: 10.1094/Phyto-61-1059.

Maize dwarf mosaic virus (MDMV) and sugarcane mosaic virus (SCMV) were partially purified by extraction of the viruses in a medium containing 0.14 m 2-mercaptoethanol, 0.17 m ascorbic acid, and 0.01 m Na diethyldithiocarbamate followed by chloroform clarification and differential ultracentrifugation. Rate-zonal, sucrose, density-gradient centrifugation was the final step in purification. On the basis of serological testing and host range studies, the various SCMV and MDMV isolates can be relegated to groups. The Johnson grass-infecting isolates of MDMV would comprise one group distinct from the isolates which do not infect Johnson grass. Sugarcane mosaic virus-A, B, D, E, and Australian SCMV (SCMV-AUS) would form another group. Sugarcane mosaic virus-H would be distinct from all other groups.