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Partial Purification and Properties of Chlorosis Inducing Toxins of Pseudomonas phaseolicola and Pseudomonas glycinea. Harry A. J. Hoitink, Assistant Professor, Department of Plant Pathology, the Ohio Agricultural Research and Development Center and The Ohio State University, Wooster 44691; S. L. Sinden, Plant Physiologist, Crops Research Division, ARS, USDA, Beltsville, Maryland 20705. Phytopathology 60:1236-1237. Accepted for publication 20 March 1970. DOI: 10.1094/Phyto-60-1236.

A chemically defined medium was used to produce high titers of exotoxins from Pseudomonas phaseolicola and P. glycinia. The exotoxins were isolated and partially purified from culture filtrates by methanol extraction, charcoal adsorption, and thin-layer chromatography. Rf values of the toxins in three different solvent systems indicated that the toxins are probably the same compound. The toxin was also isolated from soybean leaves. Results of gel filtration experiments indicated that the toxin produced in vivo is the same compound as the toxin produced in culture. This toxin caused systemic as well as localized chlorosis on soybean plants. The development of chlorosis was not light-sensitive. This toxin differs from the wildfire toxin in both chemical characteristics and biological activity.

Additional keywords: bean, soybean, exotoxin.